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. 2009 Sep 30;83(24):12680–12691. doi: 10.1128/JVI.01476-09

FIG. 2.

FIG. 2.

Knockdown of apoB and apoE expression by specific siRNAs. (A) Determination of apoB and apoE secretion by Western blotting. Huh7.5 cells were infected with HCV and then transfected with apoB, apoE, or NSC siRNA at various concentrations, using Lipofectamine RNAiMax (Invitrogen) following the manufacturer's instructions. At 24 h p.i., the levels of apoB and apoE secreted into the medium were quantified by Western blotting using apoB (Biodesign) and apoE antibodies, respectively. (B) Correlation of apoB-100 secretion and siRNA concentration. The levels of apoB-100 relative to the control (without siRNA transfection) were converted to percentages of the control (100%). The relative levels of apoB-100 (y axis) were plotted against siRNA concentrations (x axis). (C) Correlation of apoE level and siRNA concentration. The relative levels of apoE were plotted (y axis) against siRNA concentrations (x axis). The relative levels of apoB and apoE shown in panels B and C are average values for three independent experiments.