FIG. 8.

Effect of BMS-2101038 on HCV production in single-cycle HCV growth assays. Huh7.5 cells were infected with HCV at an MOI of 5 and then incubated with DMEM containing various concentrations of BMS-2101038. At 24 h p.i., the medium was collected and used to determine the levels of apoB-100 and apoE secretion and HCV production. (A) Levels of apoB and apoE determined by Western blotting. ApoB-100 and apoE in the medium were detected by Western blotting using apoB and apoE MAbs, respectively. HCV NS3 in Huh7.5 cells that were infected with medium derived from HCV-infected and BMS-2101038-treated cells was detected by Western blot analysis. (B) Correlation of apoB-100 and apoE secretion with HCV production. The levels of apoB-100, apoE, and NS3 relative to the control levels (without inhibitor treatment) were converted from the data shown in panel A and plotted against concentrations of BMS-2101038.