FIG. 7.
Peripheral nuclear aggregates in cells infected with the UL25 null virus contain pUL17 and completely assembled capsids. Hep2 cells were infected with HSV-1(F) (A to C) or the UL25 deletion virus (D to I) and were fixed and permeabilized at 14 h after infection. The cells were then reacted with a mouse monoclonal antibody, 8F5, that recognizes pentons in intact capsids or pUL17-specific IgY. The cells were then reacted with Texas Red-conjugated anti-IgY and fluorescein-conjugated anti-mouse antibodies, and images were collected with an Olympus confocal microscope. The red channel indicating the position of pUL17 is shown in the left column. The middle column displays the fluorescent signal emitted by excitation of fluorescein. A merge of these images is shown in the rightmost column. Arrows indicate nuclear aggregates that stain with both antibodies. The white bars at the bottom of panels A, D, and G correspond to 5 μm in length.