FIG. 8.
Localization of pUL17 and pUL25 in cells infected with viral mutants defective in nucleocapsid assembly. Hep2 cells were infected with the viruses indicated to the left of each row and were fixed and permeabilized at 14 h after infection. The cells were then immunostained with antibodies to pUL25, pUL17, and ICP8 as a marker of the viral DNA replication compartment. Bound immunoglobulins were recognized by the appropriate conjugates, and induced fluorescence was recorded by a confocal microscope by the use of identical settings for each image. The conjugates recognized antibodies for pUL25 (fluorescein isothiocyanate [FITC]; leftmost column), pUL17 (Texas Red; middle left column), or ICP8 (cyan5 pseudocolored white; right middle column). The rightmost column (merge) contains superimposed unaltered images from the three panels to the left. Single optical sections obtained near the middle of the cells are shown. F, HSV-1(F).