FIG. 7.

gM/ER does not alter the targeting of other viral glycoproteins. 143B cells were infected with HSV-gM/CTL (A to D) or HSV-gM/ER (E to H) at an MOI of 2 for 7 h at 37°C and then for 6 h at 20°C. The 20°C block, which specifically stops newly made proteins in the TGN, has been shown to arrest several HSV-1 envelope glycoproteins in the TGN. The cells were fixed, permeabilized, and double stained with antibodies against viral glycoprotein gB, gD, gH, or gM (green) and TGN46 (red).