FIG. 5.

Structural comparison of cofactor-bound Flaviviridae NS3 protease domains. (a) Cartoon representation of MVEV NS2B₄₅NS3pro (this study). (b) WNV NS2B₄₅NS3pro (PDB accession number 2ggv). (c) WNV NS2B₄₅NS3pro bound to Naph-KKR-H (PDB accession number 3e90). (d) HCV NS3pro₁₈NS4A (PDB accession number 1cu1). Blue, NS2B and NS4A cofactors; red, NS3pro; green, stick representation of the NS3pro catalytic triad (His-Asp-Ser); yellow, hydrophobic residues in its membrane-inserted region. Putative transmembrane helices of the cofactors NS2B and NS4A are shown as cylinders, together with their distance to the nearest visualized residue in the atomic structure.