FIG. 2.

GRP78 is upregulated in DENV-infected K562 cells and MDM. (A) K562 cells were mock or DENV infected at an MOI of 3, and at 72 h p.i., the cells were fixed and immunostained for mouse anti-DENV and anti-mouse Alexa488 (column 1) or rabbit anti-GRP78 and goat anti-rabbit Alexa647 (column 2), and images were collected by confocal microscopy. (B) MDM were generated from peripheral blood mononuclear cells derived from healthy donor blood by adherence and mock or DENV infected at an MOI of 1, and at 48 h p.i., the cells were fixed and immunostained as for panel A. (C) K562 cells were infected as for panel A, and at 72 h p.i., the cells were lysed and subjected to one-dimensional SDS-PAGE and Western analysis with rabbit anti-GRP78 with detection of complexes with goat anti-rabbit IgG-HRP conjugate and chemiluminescence. The Western blots were reprobed with mouse anti-actin and visualized with goat anti-mouse IgG-HRP and chemiluminescence.