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Supernatant from DENV-infected cells induces GRP78 in the absence of productive DENV infection. K562 cells were incubated for 10 h with supernatant (s/n) from mock-infected (A) or DENV-infected (B) K562 cells. The cells were washed, spotted onto glass slides, fixed, and costained for mouse anti-DENV (column 1) and rabbit anti-GRP78 (column 2), with detection using goat anti-mouse IgG-Alexa488 and goat anti-rabbit IgG-Alexa647, respectively, and analysis by confocal microscopy.
