FIG. 1.

Impact of AAV capsids on the transgene-specific T-cell response. CB6F1 mice were injected i.m. with 26 AAV isolates from clades A, B, C, D, E, and F, as well as with isolates that do not cluster in a particular clade (X). (A) Antigen-specific CD8⁺ T-cell responses induced by different AAV vectors expressing HIV Gag were assayed by Gag tetramer staining at 3 weeks post-vector administration. (B) HIV Gag tetramer-specific CD8⁺ T-cell responses were further analyzed for memory phenotype, and data are shown as percentages of tetramer HIV Gag-positive TEM (CD127⁺/CD62L⁻) in total CD8⁺ T cells. Data are shown as mean results with standard deviations (n = 4).