FIG. 3.

Dose response-effect of AAV prime on T- and B-cell responses. AAV8 or AAVrh32.33 expressing HIV Gag vectors were injected i.m. into CB6F1 mice (n = 4) at doses of 3 × 10⁹, 3 × 10¹⁰, and 1 × 10¹¹ GC; 8 weeks later, these mice received 1 × 10¹⁰ particles of SAdV24 Gag in parallel to a group of age-matched naïve mice. The production of CD8⁺ T-cell Gag tetramer responses from PBMCs (A and B) and anti-p24 Gag antibodies from sera (C and D) was monitored. The data are presented as the maximum response following the prime administration (peak prime), the measurement immediately prior to the boost administration (preboost), and peak response following the boost administration (peak boost). The value obtained when the same dose of SAdV24 was injected into naïve mice was called SAdV24 peak. Statistically significant differences (P < 0.05) between the peak boost and preboost data are marked with an asterisk. Data are shown as mean results with standard deviations.