FIG. 6.

HIV gp140-specific IFN-γ response following AAV-Ad prime-boost vaccine regimen in cynomolgus macaques. Cynomolgus macaques were primed i.m. with 1 × 10¹² GC of AAV8 (n = 3) (A) or AAVrh32.33 (n = 4) (B) expressing HIV W61D gp140. Ten months after the prime administration, animals were boosted with 2 × 10¹¹ particles of SAdV24 HIV gp140. A control group of two naïve cynomolgus macaques received the same dose of SAdV24 HIV gp140. Freshly isolated PBMCs were assessed for IFN-γ ELISPOT responses after in vitro exposure to peptide pools A, B, and C spanning the HIV-1 gp140 proteins at different time points. The time points of boosting with SAdV24 HIV gp140 are indicated by arrows. Each bar represents the accumulative number of IFN-γ spot-forming cells (SFC) for each of the peptide pools. PBMCs without peptide stimulation showed <50 IFN-γ spot-forming cells. Animal C10363 died at week 59 of an intestinal volvulus.