TABLE 1.
Cell expression | Results from administration of animals with: |
||||||||
---|---|---|---|---|---|---|---|---|---|
AAV8 |
SAdV24 |
AAVrh32.33 |
|||||||
% CD8 | % IFN-γ | MFI | % CD8 | % IFN-γ | MFI | % CD8 | % IFN-γ | MFI | |
Tet+/CD8+ | 18.66 ± 5.66 | 5.74 ± 1.43 | 19.13 ± 2.37 | ||||||
IFN-γ+/CD8+ | 5.87 ± 1.22 | 12.81 | 6.51 ± 0.53 | 22.12 | 17.53 ± 2.18 | 18.26 | |||
IFN-γ+ CD107a− TNF-α− IL-2−/CD8+ | 0.21 | 3.58 | 6.66 | 0.06 | 0.92 | 8.45 | 0.80 | 4.56 | 8.51 |
IFN-γ+ CD107a+ TNF-α− IL-2−/CD8+ | 3.49 | 59.45 | 8.80 | 2.33 | 35.79 | 12.83 | 8.28 | 47.23 | 11.27 |
IFN-γ+ CD107a+ TNF-α+ IL-2−/CD8+ | 1.95 | 33.22 | 15.90 | 2.97 | 45.62 | 28.87 | 8.56 | 48.83 | 25.2 |
IFN-γ+ CD107a+ TNF-α+ IL-2+/CD8+ | 0.01 | 0.17 | NA | 0.26 | 3.99 | 48.5 | 0.17 | 0.97 | 38.00 |
Splenocytes from AAV8-, SAdV24-, or AAVrh32.33-immunized mice (3 × 1010 GC; n = 4) were isolated 3 weeks postimmunization and subsequently analyzed with ICS and Gag tetramer and CD8 staining for the various cytokines and CD107a. NA, not applicable.