FIG. 3.

TAP interacts with a region of EB2 between aa 61 and 140. (A) Schematic representation of EB2Nter and the various peptides and deletion mutants used as GST fusion proteins. External boundaries of deletions are indicated in italics. (B) ³⁵S-labeled TAP was incubated with purified GST or GST fused with the overlapping peptide A, B, or C, depicted in panel A, bound to glutathione-Sepharose beads. The bound proteins were analyzed by SDS-PAGE and visualized by autoradiography. The equivalent of one-fifth of the TAP-expressing rabbit reticulocyte lysate used in each assay was loaded in the gel as an indication of the input (lanes 1, 4, and 7). (C) ³⁵S-labeled TAP was incubated with purified GST, GST-EB2Nter, or the GST-EB2Nter deletion mutants depicted in panel A, bound to glutathione-Sepharose beads. The bound proteins were analyzed by SDS-PAGE and visualized by autoradiography. The equivalent of 1/15 of the TAP-expressing rabbit reticulocyte lysate used in each assay was loaded in the gel as an indication of the input (lane 1).