TABLE 2.
Fibroblast type and passage no. | % Viral clones encoding a mammalian-type amino acid in the PB2 protein of the indicated H5N1 virus (no. of clones analyzed) |
|||
---|---|---|---|---|
A/duck/Vietnam/5001/2004 |
A/duck/Vietnam/ NCVD18/2004 |
|||
PB2-627Lys | PB2-701Asn | PB2-627Lys | PB2-701Asn | |
CEF | ||||
1 | 0 (6) | 0 (6) | 0 (8) | 0 (8) |
2 | 0 (6) | 0 (6) | 0 (8) | 0 (8) |
3 | 0 (14) | 0 (14) | 0 (8) | 0 (8) |
4 | 0 (6) | 0 (6) | 0 (6) | 0 (6) |
5 | 0 (6) | 0 (6) | 0 (6) | 0 (6) |
6 | 0 (11) | 0 (11) | 0 (10) | 0 (10) |
OEF | ||||
1 | 0 (8) | 0 (8) | 11.1 (9) | 0 (6) |
2 | 0 (8) | 0 (8) | 37.5 (8) | 0 (6) |
3 | 0 (8) | 12.5 (8) | 70.0 (10) | 0 (6) |
4 | 0 (6) | 16.6 (6) | 100 (6) | 0 (6) |
5 | 0 (6) | 16.6 (6) | 100 (6) | 0 (6) |
6 | 0 (10) | 80.0 (10) | 100 (10) | 0 (10) |
We sequenced the PB2 gene of viruses grown in OEF or CEF at the indicated passage number and calculated the proportion of viruses possessing mammalian-type amino acids at positions 627 and 701 in PB2.