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. 2009 Oct 12;29(24):6391–6400. doi: 10.1128/MCB.00337-09

FIG. 3.

FIG. 3.

Decreased Pai-2 expression is one of the causes of the increased IL-1β secretion by LPS-treated AhR KO BMDM. (A) Inhibition of IL-1β oversecretion from AhR KO BMDM by treatment with caspase-1 inhibitor (Z-YVAD-FMK) or caspase inhibitor (Z-VAD-FMK). (B) Relative expression levels of Pai-2 and Bcl-2 mRNA in AhR WT and AhR KO BMDM. (C) The effect of hPai-2 and hBcl-2 reconstitution on the LPS-induced secretion of IL-1β and TNF-α by AhR KO BMDM. BMDM from AhR KO mice were infected with the individual adenovirus (adeno) vectors and then washed and incubated for 24 h. IL-1β and TNF-α levels in the supernatants 8 h after LPS stimulation (n = 3) were determined by ELISA. (D) Assessment of hPai-2 and hBcl-2 mRNA expression in adenovirus (adeno) vector-infected BMDM by conventional RT-PCR. Error bars show standard deviations. *, P < 0.001; NS, not significant.