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. 2009 Oct 12;29(24):6391–6400. doi: 10.1128/MCB.00337-09

FIG. 4.

FIG. 4.

Arnt is not required for LPS-induced enhancement of Pai-2 expression. (A) Relative Pai-2 and AhR mRNA expression levels in AhR WT and AhR KO PEMs 4 h after treatment with (black or gray bars) or without (white bars) LPS (10 ng/ml). (B) Immunoblot analysis of Pai-2 and AhR expression in AhR WT and KO PEMs after a 16-h incubation with LPS (10 ng/ml). (C) Immunoblot analysis of Arnt in Arntflox/flox and Arntflox/flox::LysM Cre PEMs. (D) Relative Pai-2 mRNA expression levels 4 h after incubation of Arntflox/flox (black bar) and Arntflox/flox::LysM Cre (hatched bar) PEMs with LPS (10 ng/ml). (E) Left, relative expression levels of Mmp-8 and Nqo1 mRNA in Arntflox/flox (black bar) and Arntflox/flox::LysM Cre (hatched bar) PEMs treated with DMSO (white bars) or 3MC (black or hatched bar) (1 μM). Right, relative expression levels of Mmp-8 and Nqo1 mRNA in AhR WT (black bar) and AhR KO (gray bar) PEMs treated with DMSO (white bars) or 3MC (black or gray bar) (1 μM). (F) Survival of Arntflox/− (Arnt f/−; n = 7) and Arntflox/−::LysM Cre (Arnt f/−::cre; n = 12) mice after LPS challenge (25 mg/ml). Error bars show standard deviations. IB, immunoblot; +, present; −, absent; α, anti.