TABLE 2.
Plasmids used in this study
| Plasmid | Description | Reference or source |
|---|---|---|
| pRS315 | LEU2-based centromeric plasmid | 44 |
| p1172 | pGBT9; two-hybrid vector with Gal4-DBD | Clontech |
| p1173 | pGAD424; two-hybrid vector with Gal4-AD | Clontech |
| p1366 | PRM5 (YIL117c)-lacZ with URA3 marker | 21 |
| p2052 | FKS2 (−540 to −375)-CYC1-lacZ with URA3 marker | 27 |
| p2066 | CLN2 (−600 to −400)-CYC1-lacZ with URA3 marker | 27 |
| p2266 | pGBT9 MPK1(1-328) | 26 |
| p2313 | YEp351 MPK1-FLAG | 26 |
| p2316 | YEp351 mpk1-T190A, Y192F-FLAG | 27 |
| p2317 | YEp351 mpk1-K54R-FLAG | 27 |
| p2352 | pGAD424 SWI4(1-1093) | 27 |
| p2415 | pUT36; URA3-based MET25 promoter | 27 |
| p2418 | pUT36 SWI4-6HIS | 27 |
| p2704 | pRS315 MPK1-FLAG | This study |
| p2705 | pRS315 mpk1-H116A-FLAG | This study |
| p2706 | pRS315 mpk1-R196A-FLAG | This study |
| p2707 | pRS315 mpk1-K234A-FLAG | This study |
| p2708 | pRS315 mpk1-D237A-FLAG | This study |
| p2709 | pRS315 mpk1-K83A-FLAG | This study |
| p2710 | pRS315 mpk1-Q166A-FLAG | This study |
| p2711 | pRS315 mpk1-W321A-FLAG | This study |
| p2712 | pRS315 mpk1-K54R-FLAG | This study |
| p2713 | pRS315 SWI4 | This study |
| p2714 | pRS315 swi4-(I913A, I915A) | This study |
| p2715 | pRS315 swi4-(L966A, L968A) | This study |
| p2716 | pUT36 swi4-(I913A, I915A)-6HIS | This study |
| p2717 | pGAD424 SWI4(1-671) | This study |
| p2718 | pGAD424 SWI4(672-1093) | This study |
| p2824 | YEp351 mpk1-S423A-FLAG | This study |
| p2825 | YEp351 mpk1-S428A-FLAG | This study |
| p2826 | YEp351 mpk1-S423A, S428A-FLAG | This study |