FIG. 3.

FANCD2 focus formation is impaired in XPF-ERCC1-deficient cells. Wild-type (C5RO) and XP-F (XP51RO) human fibroblasts were seeded on glass coverslips. Sixteen hours later, the cells were exposed to 3 μM MMC for 1 h and then fixed at multiple time points. FANCD2 was detected by immunofluorescence. Cells with FANCD2 foci were counted. Representative images are shown, and the averages from three experiments are plotted. A paired Student's t test was used to calculate significance. DAPI, 4′,6′-diamidino-2-phenylindole.