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Electrostatic free energy of cations along the cytoplasmic pore of Vp-ZntB. The four different locations along the pore are described in the text. The different curves correspond to monovalent (Q = +1) or divalent (Q = +2) pore ions and the presence of Cl− in the protein structure (x-ray Cl−). Ionic screening corresponding to 150 mM salt was included inside the wide cytoplasmic pore.
