Table I.
Analysis of Alignment Tensor Parameters Based on RDCs Measured for DH-PH and DH-PH/RhoA Complex
| Q [%] | Da [Hz] | α | β | γ | N | |
|---|---|---|---|---|---|---|
| DHa | 36.1 ± 2.6 | 12.1 ± 0.4 | 59.6 ± 1.1 | 20.5 ± 1.7 | −54.5 ± 16.5 | 35 |
| PHa | 32.6 ± 2.1 | 13.1 ± 0.5 | 52.4 ± 1.3 | 18.5 ± 1.2 | −75.8 ± 2.6 | 34 |
| DH-PHa | 37.2 ± 1.7 | 12.5 ± 0.3 | 56.0 ± 0.8 | 19.0 ± 0.9 | −71.7 ± 3.9 | 69 |
| DH-PH/RhoAb | 39.0 ± 2.3 | 16.5 ± 0.6 | 57.5 ± 2.3 | 19.3 ± 1.2 | −78.5 ± 3.2 | 37 |
Calculations of Q-factors (Q), magnitude of the alignment (Da) and Euler angles (α, β, γ) defining rotations of molecular coordinates about x, y, and z axes, relative to principal axes frame of alignment tensor were carried out employing program PALES31 based on the crystal structure of DH-PH/RhoA complex (PDB code 1XCG, molecule A) and using N experimental 1DHN RDCs (see Materials and Methods for details).
a
The RDCs measured for DH-PH were fit to coordinates of separate DH, PH domains, and DH-PH tandem.
b
The analysis of RDCs measured for DH-PH in the complex with RhoA.