Figure 7.

Distance analysis for the eight pairs of cross-polarized nuclei in TC5b (Trp6 to Ile4, Leu7, Prol12, and Argl6, Table 1 of Ref. 25 for the 6M urea simulations at different temperatures: black at 278 K, red at 360 K, and blue at 298 K. The green horizontal line in each panel corresponds to the specific distance assigned to the cross-polarized nuclei in the native state (i.e., taken from the set of NMR model structures in aqueous solution at 278 K,⁷ and the magenta horizontal line corresponds to the NOE contact distance between the cross-polarized nuclei in the 6M urea-denatured state of TC5b derived from photo-CIDNP experiments at 278 K.