Table X.
I50 (Affinity) (M) | LoDa (Sensitivity) (M) | SE (M) | |
---|---|---|---|
IL | 4.02 × 10−7 | 1.00 × 10−8 | 2.44 × 10−8 |
S268C | 3.76 × 10−8 | 1.00 × 10−9 | 1.77 × 10−9 |
The LOD was determined on the basis of 99% confidence intervals, which, assuming a normal distribution, corresponds to 2.6 × standard error of the measurement (σ). Using then the modified relationship for the Langmuir absorption isotherm, LOD can be calculated as LOD = 2.6 × σ × I50/(100 − 2.6 × σ) as reported by Koblizek et al.29