Figure 6.

Binding of coexpressed mutated polypeptides. (A) Protection of H68h by GST-k72b′. Arrowheads indicate the migrations of GST-k72b′, ΔTPR1, ΔTPR4, and Δh120 polypeptide. Lysates coexpressing H68h were incubated with Ni-NTA beads and the bound polypeptides were separated by SDS PAGE. (B) Amino acid residues in H68h required for binding to GST-k72b′. Analysis of lysates coexpressing GST-k72b′ with H68h or H68h mutant derivatives (as indicated) on Ni-NTA beads (Ni) or glutathione Sepharose 4B (GST). The arrowhead points to the 5.2 kDa wildtype or mutant H68h polypeptides.