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Kinetic Analysis of wt. AtuSFGH with Various Substrates
| Km (M) | Vmax (moles min−1 mg−1) | kcat (s−1) | kcat/Km (M−1 s−1) | |
|---|---|---|---|---|
| pNP-acetate | 0.48 ± 0.045 | 3.66 ± 0.11 | 1.95 | 4.07 × 103 |
| pNP-propionate | 0.05 ± 0.006 | 3.46 ± 0.12 | 1.84 | 3.85 × 104 |
| pNP-butyrate | 0.11 ± 0.007 | 8.40 ± 0.14 | 4.48 | 4.08 × 104 |
| pNP-caproate | 0.02 ± 0.002 | 1.26 ± 0.033 | 0.67 | 3.96 × 104 |
| α-naphthyl propionate | 0.35 ± 0.027 | 3.57 ± 0.19 | 1.90 | 5.54 × 103 |
| α-naphtyl butyrate | 0.44 ± 0.040 | 3.24 ± 0.21 | 1.73 | 3.93 × 103 |
