Figure 3. Identification of naturally processed arenavirus-derived peptides in HLA-A*1101 transgenic mice following peptide immunization.

HLA-A*1101 transgenic mice were immunized s.c. with one of the listed peptides. After 11 to 14 days, splenocytes were restimulated for 6 days with the immunizing peptide, and CD8⁺ T cells were isolated. Effector CD8⁺ T cells were exposed to human BVR cells that had been either pulsed with 1 µg/ml of peptide, or infected with the appropriate rVACV in an ex vivo IFN-γ ELISPOT assay. The dotted line indicates the threshold for peptide positivity (net SFC/10⁶ cells ≥20). Error bars indicate SD. Results are representative of at least two independent experiments.