Fig. 8.

Soluble αvβ6 and αvβ8 trigger fusion of CHO-K1 cells transfected with gB and gHgL but solubleαvβ3 does not. Cells were transfected with gB and gHgL, overlaid with integrins, and 20 h later were fixed and stained with monoclonal antibody to gB. (A) Cells overlaid with αvβ3. (B) Cells overlaid with αvβ6. (C) Cells overlaid with αvβ8. (D) Percentage of cells expressing gB that contained 4 or more nuclei (% fusion) after overlay with integrins (black bars) or integrins preincubated with cognate blocking antibody (gray bars). Error bars indicate SD of 5 experiments without antibody and 2 experiments with antibody.