Table 1.
RNA-binding analyses of FBF-2
| RNA | RNA sequence* | Kd (nM) | Krel† |
|---|---|---|---|
| 123456789 | |||
| gld-1 FBEa | UGUGCCAUA | 33.2 ± 1.5 | 1.0 |
| gld-1 FBEa G4A | UGUACCAUA | 39.4 ± 1.3 | 1.2 |
| FBE | UGUACUAUA | 45.8 ± 3.2 | 1.4‡ |
| fem-3 PME | UGUGUCAUU | 52.9 ± 2.4 | 1.6‡ |
| PME_U9A | UGUGUCAUA | 56.7 ± 3.4 | 1.7‡ |
| PME_U9C | UGUGUCAUC | 81.3 ± 3.5 | 2.4‡ |
| ced-4 FBEa | UGUACAAUA | 73.6 ± 1.7 | 2.2‡ |
| mpk-1 FBEb | UGUAAUAUA | 81.7 ± 2.8 | 2.5‡ |
| fog-1 FBEa | UGUAAAAUC | 96.9 ± 3.4 | 2.9‡ |
| gld-1 FBEb | UGUGUUAUC | 127 ± 6.4 | 3.8‡ |
| NRE | UGUAUAUA | >1,000§ | >30‡ |
*Numbers indicate positions in the core recognition sequence. Bases that differ from the sequence of gld-1 FBEa are shown in boldface.
†Krel reports the affinity of FBF-2 for the RNA sequence relative to the affinity of FBF-2 for gld-1 FBEa RNA. Double daggers (‡) denote statistically significant differences (P < 0.05).
§Saturable binding could not be achieved at the highest protein concentration; therefore, we report a lower limit for the Kd.