Fig. 1.

Proteomic analysis of endogenous Piwi complexes identifies Tudor domain family proteins as physiological binding partners. (A) Hierarchical clustering of proteins identified by 2 independent immunoprecipitations (IP) of Miwi, Mili, and corresponding IgG controls through tandem mass spectrometry. Proteins for which 3 or more peptides were identified are shown. Proteins that interact specifically with Miwi or Mili are illustrated in blue and red boxes, respectively. The cyan box indicates common proteins associated with both Miwi and Mili. (B) Identification of multiple germline Tudor domain proteins in Miwi and Mili complexes. Miwi and Mili protein interaction networks are shown with baits in blue and Tudor domain proteins in orange. Proteins represented by 3 or more peptides and not present in the IgG control IP, and proteins represented by peptides with a 5-fold increase over the IgG control IP are shown.