Impaired memory formation in GCV-treated Nestin-tk transgenic mice in the standard version of the hidden platform MWM. A, The experimental scheme. B, Neither latency nor distance swum to reach the hidden platform during training was significantly different between tg-GCV and wt-GCV (latency: ANOVA, F(1,30) = 2.179, p > 0.15; distance: ANOVA, F(1,30) = 0.566, p > 0.46; n = 16 for each group). C, Short-term retention from daily probe tests from day 4 to day 8 of training. Notice that tg-GCV mice spent progressively less time in the target quadrant whereas wt-GCV mice spent progressively more time there. D, Long-term retention from the probe test 1 week after training. tg-GCV mice spent equal time in all quadrants whereas wt-GCV mice spent significantly more time in the target quadrant. The red dotted line indicates chance level (15 s). The hidden platform (black square) is located in the northwest quadrant. *p < 0.05 between the target quadrant and all other quadrants; for detailed statistical data for C and D, see supplemental Table 2, available at www.jneurosci.org as supplemental material. Error bars represent ± SEM.