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. Author manuscript; available in PMC: 2009 Dec 2.
Published in final edited form as: Anticancer Res. 2007 Jul–Aug;27(4B):2175–2183.

Figure 3.

Figure 3

Silvestrol induces apoptosis in LNCaP cells and the general caspase inhibitor, Boc-D-Fmk, inhibits silvestrol-induced apoptosis. (A) The TUNEL assay was used for quantification of silvestrol-induced apoptosis. LNCaP cells were treated with 30 nM or 120 nM silvestrol for 24 h, harvested, fixed, stained with FITC-dUTP and analyzed by flow cytometry as detailed in “Material and Methods.” (B) Immunoblot of PARP cleavage with treatment of silvestrol (30 nM or 120 nM). (C) LNCaP cells were treated with 30 nM or 120 nM silvestrol for 24 h in the absence or presence of 50 μM Boc-D-Fmk. An antibody specific for cleaved PARP was used.