Figure 6. Assessment of CCRF-CEM cell viability by propidium iodide (PI) and annexin V labeling following treatment with CCR4 antagonists.

Propidium iodide (PI) labels cells lacking intact plasma membranes and hence at an advanced stage of cell death. Annexin V labels cells at early and late stages of apoptosis. Plots show representative data for cells cultured in medium alone or in medium to which solvent (DMSO) or antagonist has been added. Numbers indicate percentage of cells in each quadrant.