Figure 7. CCR4 antagonists enhance cellular and humoral responses in mice.

(a) Effect of SP50 on T cell priming to Mycobacterium tuberculosis protein antigens Rv1818c or Rv3812. Splenocytes (0.5×10⁶) from mice immunised with the indicated proteins+SP50 or DMSO were cultured in vitro in the presence of 5 µg/ml of either Rv1818c or Rv3812 recombinant proteins for 5 days. Cells from three mice per group were tested individually in quadruplicate wells. Antigen-specific T cell proliferation was measured by thymidine incorporation assay and data are presented as stimulation index (SI, mean cpm of the antigen stimulated wells/mean cpm of control wells). Significant differences are indicated by asterisks * p<0.05. The results are from one experiment. (b) IgG serum antibody responses against MSP-1₁₉ measured by ELISA 14 days post vaccination with Ad-MSP-1₄₂ plus the indicated compounds or DMSO control. Three mice per group were tested individually. Columns represent the mean±SD. Significant differences are indicated by asterisks * p<0.05. Similar results were obtained in two independent experiments.