Table 2.

Dissociation constants (K_D) of mAbs to the MIR for α1/α7 and α1/AChBP chimeras

mAbs	KD (nm)
	α1 AChR	α1(66–76)/α7	α1(60–81)/α7	α1(2–14, 66–76)/α7	α1(2–14, 60–81)/α7	α1(1–32, 60–81)/α7	α1(1–30, 60–81)/AChBP	α1(1–30, 60–81, 107–115)/AChBP
Rat 210	0.127 ± 0.028	80.2 ± 54.3	0.354 ± 0.111	0.028 ± 0.012	0.031 ± 0.005	0.050 ± 0.007	0.169 ± 0.020	0.18 ± 0.02
Rat 198	12.7 ± 1.5	3860 ± 1570	48.9 ± 40.1	0.109 ± 0.037	0.068 ± 0.013	0.183 ± 0.097	2.50 ± 0.15	3.19 ± 0.14
Rat 35	2.06 ± 1.15	None	2370 ± 820	0.306 ± 0.717	0.026 ± 0.016	0.034 ± 0.010	0.140 ± 0.036	0.297 ± 0.010
Rat 192	0.0071 ± 0.0007	None	None	None	None	None	0.137 ± 0.025	0.121 ± 0.014
Human 637	0.0050 ± 0.0012	None	None	None	None	0.030 ± 0.023	0.075 ± 0.013	0.090 ± 0.016

Binding of different concentrations of mAbs to ¹²⁵I αBgt labeled α1/α7 chimeras or ¹²⁵I labeled α1/AChBP chimeras was determined by radioimmunoassay. The averages of duplicate determinations were fitted by the Hill equation (R = 1/(1 + 10^{(LogK_D − Log[ACh]) × Hill slope)}) to determine K_D values. The single-fit errors are shown.