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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Plant J. 2009 Feb 3;58(5):831–842. doi: 10.1111/j.1365-313X.2009.03816.x

Figure 6.

Figure 6

Neither Alb3 nor SecY are necessary for cpTatC integration. (a) Arabidopsis seedlings were germinated on MS media, grown, and harvested as described (Experimental Procedures). Membranes were isolated from seedlings and samples of equal mass tissue were analyzed by SDS-PAGE and immunoblotting. (b) Isolated membranes were also dissolved in 2% digitonin and analyzed by BN-PAGE and immunoblotting using antibodies to atcpTatC. (c) Membranes were subjected to alkaline carbonate (-CO3) or mock extraction (IB). Supernatants (S) and membrane pellets (P) from each extraction were analyzed using SDS-PAGE and immunoblotting. Thylakoids (Thy) prepared from isolated Arabidopsis chloroplasts were analyzed in the far right lanes of each SDS-PAGE gel to provide reference bands for the other lanes. (*) Non-specific bands. All antibodies were applied at 1:5000.