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Cell proliferation and metabolic activity. Incubation of fibroblast-like synovial cells (FLS) for 16 hours with 5 mg/ml advanced glycation end products-modified (AGE)-BSA significantly reduced cell proliferation as measured by incorporation of bromodeoxyuridine (BrdU; P < 0.01, n = 6). Determination of metabolic activity in FLS with the MTT assay. AGE-BSA induced a significant decrease in metabolic activity of FLS (*P = 0.01, n = 6).
