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Quantification of cell death. (a) Percentage of vital cells as measured by FACS analysis (annexin-V and propidiumiodide negative). Incubation of cells with advanced glycation end products-modified (AGE)-BSA significantly reduced the number of vital cells from day three (*P < 0.05, n = 4). (b) Percentage of necrotic and late apoptotic cells (annexin-V and propidiumiodide positive) increased three to seven days during treatment with 5 mg/ml AGE-BSA (*P < 0.05, n = 4).
