TABLE 1.

PCR primers used for detection of mRNA in myotubes derived from neonatal rat primary culture and mouse C₂C₁₂ cell line

mRNA	Forward (5′–3′)	Reverse (5′–3′)	PCR product
			bp
Rat samples
P2Y1	CCCTAACTATGATGCAGCTT	GCTGCATCTTTATCACCCTG	151
P2Y2	GGCCCGAGAGCTCTTTAGC	GCAAAGCGGACCAGTCTCT	386
P2Y4	GGGGACAAGTATCGAAACCA	GCCCCTGCAGTTAGTTCCCTT	208
P2Y6	GACACCTGTGTTTCGGGGAC	CCTCTACAGGAGGGGCCTT	259
P2Y11	ACTGGTGGTTGAGTTCCTGG	TCAGGTGGGAGAAGCTGAGT	410
P2Y12	CAGAAATTCCTTGATGAGCA	ATGTGGTGATTTCCTTGGAG	175
P2Y13	TGTGCACTTTCTCATCCGTG	TTGCCAGGAAGAGAGTTG	570
P2Y14	CGCAATATATTCAGCATTGTGC	TCAGGAAAGCACAGATACTTTG	317
P2X1	GTTCAGCATGAAGACAGGCA	GTATAGATGTGTGAGGGGCC	136
P2X2	TGTGACTGGGAAACAGAAACC	AGGAGATGGCAGGGAACC	114
P2X3	AAGAAGGGCTGCTATTTCTGC	AGGCATGCAAGGGGTAAAG	126
P2X4	TCTGGTGTGCTGTTGGCTGG	ACCTGAGAGAGCCTCCTTCC	152
P2X5	ACTTAGGGAAGAGCAAACTCC	AGCAAGAGCTGAACTGCACA	156
P2X6	AGGCTAGGGTGAAAGCAACA	GCAGGAATATCAGGTTCTTTGG	201
P2X7	TAAAGTTTGGATGTGGCTTGG	TCTGTGTGGTGTGTGGTGTG	156
IL-6	CCAATTTCCAATGCTCTCCT	ACCACAGTGAGGAATGTCCA	190
c-fos	AGGCCGACTCCTTCTCCAG	CAGATAGCTGCTCTACTTTGC	298
GAPDH	CAACTTTGGCATTGTGGAAG	CTGCTTCACCACCTTCTTG	295
Mice samples
P2Y1	TGGCGTGGTGTACCCTCTCAAGTC	CGGGACAGTCTCCTTCTGAATGTA	558
P2Y2	CTGGAACCCTGGAATAGCAC	GCTGGTGGTGACGAAGTAGA	513
P2Y4	AGCCCAAGTTCTGGAGATGGTG	GGTGGTTCCATTGGCATTGG	492
P2Y6	CACCTGTGATTTGGCAACTG	TCTTGGCAAATGGATGTGAA	336
P2Y11	ACTGGTGGTTGAGTTCCTGG	TCAGGTGGGAGAAGCTGAGT	410
P2Y12	CACCTCAGCCAATACCACCT	AACATGAAGGCCCAGATGAC	453
P2Y13	GAAGAGAGGCACATGCAACA	TTACTAATGCCAGGCCAACC	345
P2Y14	CAGTGCATGGAGCTCAAAAA	GCAGCCGAGAGTAGCAGAGT	347
P2X1	CATTGTGCAGAGAACCCAGAA	ATGTCCTCCGCATACTTGAAC	776
P2X2	ACGTTCATGAACAAAAACAAG	TCAAAGTTGGGCCAAACCTTTGG	360
P2X3	CTGTATATCAGACTTCTTCACCTACGA	TTATGTCCTTGTCGGTGAGGTTAG	596
P2X4	GAGAATGACGCTGGTGTGCC	TTGGTGAGTGTGCGTTGCTC	356
P2X5	TCCACCAATCTCTACTGC	CCAGGTCACAGAAGAAAG	400
P2X6	CGATTCACTCTCCAGTCCG	GGTCCTCCAGTAGAAACCG	427
P2X7	AAGTCTCTGCCTGGTGTC	GGCATATCTGAAGTTGTAGC	401