TABLE 2.

Effect of several blockers over maximal fast and slow calcium signal evoked by tetanic stimulation (45 Hz, 400 pulses, 1 ms each)

Data were analyzed using Dunnett's t test for one-tail comparison between several treatments and control. n, number of cells analyzed. Controls are derived from 17 different cultures. For treatments, cells correspond to 3–5 different cultures. NS, not significant.

Blocker	Fast calcium signal	p value	Slow calcium signal	p value	n
	% maximal value		% maximal value
Control	100.2 ± 3.4		100.1 ± 5.5		282
2 units/ml apyrase, 7 min	98.7 ± 8.1	NS	21.8 ± 7.2	<0.05	13
2 units/ml apyrase, 30 min	83.1 ± 9.7	NS	22.1 ± 5.7	<0.01	16
2 units/ml apyrase, 60 min	62.2 ± 6.9	<0.05	15.3 ± 6.5	<0.01	20
100 μm suramin, 20 min	47.5 ± 7.2	<0.01	36.3 ± 8.1	<0.01	43
0.1 μm MRS2179, 20 min	82.8 ± 2.7	<0.01	68.4 ± 7.2	<0.01	139
100 μm oleamide, 20 min	61.6 ± 10.2	NS	61.0 ± 25.9	NS	15
100 μm oleamide, 30 min	35.8 ± 6.0	<0.05	8.8 ± 3.2	<0.05	17
100 μm anti-Pnx1, 20 min	49.4 ± 7.7	<0.01	53.8 ± 9.6	<0.05	25
100 μm anti-Pnx1, 30 min	42.0 ± 7.0	<0.01	27.9 ± 7.0	<0.01	24