TABLE 1.
Free and esterified jasmonates in healthy and wounded Arabidopsis leaves
Amounts of free and bound jasmonates were determined semi-quantitatively in unwounded leaves (UNWND), wounded leaves (WOUNDED) 3 min after wounding and in leaves distal to the wound (W DISTAL) at the same time point for WT and the lox2-1 mutant. The abundance of the different jasmonates was obtained from integrated peak areas of extracted ion chromatograms, which are given as ± S.D. (n = 3). ND, not detected. Lyso-arabidop, sn2-O-(dinor-oxophytodienoyl)-monogalactosyl monogylceride (25).
| WT |
lox2-1 |
|||||
|---|---|---|---|---|---|---|
| UNWND | WOUNDED | W DISTAL | UNWND | WOUNDED | W DISTAL | |
| JA | ND | 20.9 ± 10.4 | 11.8 ± 3.5 | ND | 16.0 ± 6.9 | 5.2 ± 2.8 |
| OPDA | 16.5 ± 5.9 | 246 ± 61 | 50.1 ± 13.3 | 18.0 ± 5.8 | 103 ± 43 | 22.1 ± 6.5 |
| dinor-OPDA | ND | 65.6 ± 18.1 | 3.8 ± 0.5 | ND | 12.1 ± 4.4 | ND |
| Arabidopside A | 166 ± 16 | 4482a ± 646 | 393 ± 140 | ND | 29.3 ± 28.1 | ND |
| Arabidopside B | 28.3 ± 2.3 | 2923 ± 610 | 77.3 ± 10.0 | ND | 5.2 ± 6.5 | ND |
| Arabidopside C | 3.2 ± 0.7 | 967 ± 460 | 8.3 ± 3.5 | ND | 3.2 ± 3.0 | ND |
| Lyso-arabidop | 9.6 ± 4.6 | 2310 ± 402 | 25.4 ± 9.9 | 3.2 ± 2.6 | 56.2 ± 28.8 | 5.9 ± 2.2 |
a This value is a minimum because of detector saturation.