FIGURE 2.

Activation of purified PKCϵ by PKC activators. Purified PKCα, βII, γ, δ, or ϵ (9 ng) was preincubated for 5 min at room temperature with DHA-CP6 (A), EPA-CP5 (B), AA-CP4 (C), or DCP-LA (D) followed by PKC activity measurement as described under “Experimental Procedures.” E, activation of purified PKCϵ by other cyclopropanated and epoxidized fatty acids, alcohols, and methyl esters. Samples were preincubated for 5 min at room temperature with purified PKCϵ (500 ng) followed by PKC activity measurement. Values are x̄ ± S.E., two-tailed Student's t test. *, p < 0.02; **, p < 0.001.