FIGURE 4.

Decreased intracellular levels of Aβ in cells (A) and in culture medium (B) from cells exposed to PKC activators. Neuro2a neuroblastoma cells transfected with human APPSwe/PS1Δ9 (23, 24), growing in 6-well plates, were incubated with low serum (1%) medium for 24 h. The medium was replaced with fresh low serum medium and various concentrations of bryostatin, DCP-LA, or DHA-CP6 in 10 μl of ethanol, or 10 μl of ethanol alone was added. After 18 h, the cells were washed with phosphate-buffered saline and scraped into 200 μl of homogenization buffer, homogenized, and Aβ was measured by ELISA. The medium was collected, and Aβ was isolated by adsorption onto reversed phase columns (Waters C₁₈ Sep-Pak cartridges), washed, and eluted with acetonitrile followed by ELISA measurements.