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. 2009 Oct 15;284(50):34531–34537. doi: 10.1074/jbc.M109.046102

FIGURE 2.

FIGURE 2.

Rab39a is required for IL-1β secretion. A, knock down of Rab39a is shown by PCR (upper panel). THP1 cells were transfected with siRNA to Rab39a for 48 h. Cells were stimulated with 100 ng/ml LPS overnight before harvesting. IL-1β and TNF-α levels were measured by ELISA. B, PBMCs were transfected with siRNA to Rab39a for 48 h. Cells were stimulated with 100 ng/ml LPS overnight before harvesting. IL-1β and TNF-α levels were measured by ELISA. A and B, results are expressed as mean ± S.D. for triplicate determinations. ***, p < 0.005; *, p < 0.05. All results are representative of three separate experiments. C, RNA from THP1 cells from siRNA experiments was extracted, and mRNA levels of IL-1β were measured by real time PCR. Expression of IL-1β mRNA was normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA. Data were calculated as fold change and are presented relative to that of untreated controls. Results shown are each representative of at least three independent experiments.