FIGURE 4.

Rab39a can be induced by pro-inflammatory stimuli. A, Rab39a promoter contains numerous putative transcription factor binding sites as indicated (adapted from the Genomatix website). B, THP1 cells were stimulated with 100 ng/ml LPS or 5 nm Malp-2 or 1 μg/ml Pam₃Cys for the indicated times. mRNA levels were measured by real time PCR with primers specific for Rab39a and Rab39b; expression is normalized to that of glyceraldehyde-3-phosphate dehydrogenase and is presented relative to that of untreated controls. Results shown are representative of three independent experiments.