FIGURE 3.

Plin regulates the molecular interaction of Abhd5 and Atgl in Cos7 cells. A, forskolin (FSK)/IBMX increased the interaction of Abhd5 and Atgl in cells expressing wild type (WT) Plin (p < 0.001), and this effect was absent in cells expressing phosphorylation-defective Plin (Δ1–6) and in cells expressing mutant Abhd5 that has defective interactions with Plin. Values are the means ± S.E. of three independent experiments, each performed in triplicate. PC, protein complementation. B, combined mutation of PKA sites 5 and 6 of Plin disrupted PKA-mediated regulation of Abhd5-Atgl interactions, but individual mutations did not. Values are the means ± S.E. of three independent experiments, each performed in triplicate.