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. 2009 Oct 16;284(50):34545–34552. doi: 10.1074/jbc.M109.056499

FIGURE 2.

FIGURE 2.

MTA1 is an upstream stabilizer of p53. A and B, protein extracts from MTA1+/+ and MTA1−/− MEFs (A) or from the MTA1−/− and MTA1−/−/V5-MTA1 MEFs (B) were subjected to Western blot analysis with the indicated antibodies. C, qPCR analysis of p53 mRNA levels in the MTA1+/+, MTA1−/−, and MTA1−/−/MTA1 MEFs. D and E, Western blot analysis of MTA1 and p53 protein expression in the U2OS cells transfected with increasing amounts of specific siRNA targeting human MTA1 or control siRNA (D) or transfected with increasing amounts of Myc-MTA1 expression plasmids (E). F, H1299 cells were transfected with the pCMV-p53 plasmid either alone or in combination with increasing amounts of Myc-MTA1, and the expression of MTA1 and p53 proteins was detected by Western blot analysis.