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Phosphorylation and subcellular localization of RBM4 during myoblast differentiation. A, immunoblotting of cell lysates prepared from C2C12 cells that were cultured in growth (GM) or differentiation medium (DM) for 2 days. Relative level of myosin heavy chain (MHC) protein expression and RBM4 phosphorylation in GM versus DM is indicated below the gels. B, immunofluorescence of C2C12 cells that transiently expressed FLAG-RBM4 and were cultured in GM or DM for 1–3 days.
