FIGURE 1.

Domain architecture of M. tuberculosis Rv0019c and amino acid sequence alignment with FHA domains of GarA and EmbR. A, a schematic representation of Rv0019c domain organization. TM, trans-membrane region; SP, signal peptide. The domains of Rv0019c were predicted using Smart and Interproscan. B, the alignment was performed using ClustalW2. Six conserved residues in the FHA core homology region are boxed and marked with a star. The seven conserved residues found in >65% of FHA domains are shown above the alignment. The residues chosen for mutagenesis are indicated by an arrow. Secondary structural elements of EmbR (34) are shown below the alignment.