FIGURE 2.

HC-IC association in the ΔrobA mutant and the ΔnudG(LC8) mutant at 42 °C. A, association between HC and IC is not apparently disrupted in ΔrobA cells as S-IC was able to pull down HC at both 32 °C (top panel) and 42 °C (middle panel), similar to WT cells. B, S-IC was also able to pull down HC in the ΔnudG mutant grown at 42 °C (bottom panel). In this experiment, the GR5 strain without S-IC was used as a negative control. C, quantitative analysis on the ratios of HC to S-IC after purification. Values were all relative to the wild-type values, which were set at 1. Mean and S.D. values were determined from three independent experiments. Note that there is no significant difference between the mean value in either mutant and that in the wild type at a p value of 0.05.