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. 2009 Oct 16;284(50):34760–34768. doi: 10.1074/jbc.M109.026872

FIGURE 4.

FIGURE 4.

Rescue of the nudN117 mutant phenotype by the LIC-coding gene and a biochemical analysis of the nudN117 mutant of LIC. A, colony phenotypes of the nudN117 mutant, a nudN117 strain rescued by the LIC gene and a wild-type control strain. The strains were grown on a YUU plate at 42 °C for 2 days. B, DAPI staining showing that the LIC gene allowed normal nuclear distribution in the nudN117 mutant. Strains were grown in YUU liquid medium overnight at 42 °C. C, Western blot showing that the HC proteins could no longer be pulled down by the S-IC in the nudN117 mutant grown at 42 °C. WT cells and nudR825, a ts mutant of p62 of the dynactin complex (38), were used as controls. D, quantitative analysis on the ratios of HC to S-IC after purification. Values were all relative to wild-type values, which were set at 1. Mean and S.D. values were determined from four independent experiments. Note that the mean value of the nudN117 mutant is significantly different from that of the wild type (p < 0.001).