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MAGE-11 and TIF2-dependent increase in AR AF1 activity. A, CV1 cells were transfected with 5 μg of PSA-Enh-Luc and 0.05 μg of pCMV5 empty vector (p5) or pCMV-AR-(1–660) in the absence and presence of 0.1 μg of pSG5-MAGE and/or 2 μg of pSG5-TIF2. B, CV1 cells were transfected with 5 μg of PSA-Enh-Luc and 0.05 μg of p5 or 0.05 μg of pCMV-AR-(1–660) in the absence and presence of 2 μg of pSG5-TIF2 and/or 1 μg of pSG5-MAGE WT or the indicated F-box mutant.
